Simultaneous coadministration of omeprazole (20 mg once daily) and ledipasvir/sofosbuvir (90/400 mg single dose) decreased ledipasvir Cmax and AUC by 11% and 4%. Sofosbuvir Cmax increased by 12% and AUC was unchanged; Cmax and AUC of GS-331007 increased by 14% and 3%. Omeprazole 20 mg can be administered simultaneously with ledipasvir/sofosbuvir but should not be taken before ledipasvir/sofosbuvir.

Coadministration of omeprazole (20 mg once daily) simultaneously with ledipasvir/sofosbuvir (90/400 mg single dose) was studied in 16 subjects. Simultaneous coadministration decreased ledipasvir Cmax and AUC by 11% and 4%, increased sofosbuvir Cmax by 12% with no effect on AUC, and increased GS-331007 Cmax and AUC by 14% and 3% (n=16). When omeprazole (20 mg once daily) was administered 2 h prior to ledipasvir alone (30 mg single dose), ledipasvir Cmax and AUC decreased by 48% and 42% (n=17). Omeprazole 20 mg or lower can be administered simultaneously with ledipasvir/sofosbuvir under fasted conditions.